Precipitin: The soluble antibodies behave like precipitins, which react with the soluble antigens and bring a precipitate formation in the form of a visible insoluble mass.There are specific terms, which we should know before proceeding to the theory of precipitation reaction. Precipitin is an antibody which reacts with a respective antigen to form a “ Precipitate”. The lattice formed by antigen and antibody is visible, either in the form of precipitin ring or in the way of precipitin line. The antigen and antibody react with each other to form an immunocomplex or lattice at the zone of equivalence (Abs and Ags are at equilibrium). both antigen and antibody diffuse either in one dimension or two dimensions in liquid or semi-solid media. Precipitation in Agar by ImmunodiffusionĪ precipitation reaction is an immuno or serological assay where the two reactants, i.e.Here, we will focus on the definition, principle and methods of the precipitation reaction in solution and agar (by diffusion and electrophoresis) along with the meaning of some important terms relative to this context. Thus, the end product appears as a precipitate, which can be measured by devices like turbidometer and nephelometer. ![]() For the reaction to occur, the concentration of both antigen and antibody must be equal, which in turn produce a visible mass of precipitate or lattice. If anyone’s concentration (either antigen or antibody) exceeds, the immunocomplex will not form.
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